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Such surprises as the odd hardware mentioned previously keep life interesting. And advising against the unknown is, at best, tricky. But the other half of this equation entering the job with the right tools to begin with I ve got covered. I ll start with your soldering iron. I ve used em all. The mini butane soldering irons are nice for electronic repairs in the field, away from electric power. Big soldering guns are fine for larger solder connections than you ll be doing here. Stand-alone soldering pencils are fine if you file down the tips to a small beveled point. But what I ve found to be best, and what you ll be using here, is a low-wattage (25 40 watt) soldering station. This consists of a soldering pencil, pencil rest, and tip-cleaning sponge. A small tip is a must. It s also nice to have an adjustable temperature station, one that allows you to adjust tip temperature, but as long as the unit is made for small electronic work, you ll be okay. I use two Weller stations, the WTCPT (my main station) and the smaller WLC100. They re reasonably priced and both are workhorses (see Figure 5-1). Each comes with cleaning sponge and pencil (iron) rest.

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The yields stood at 9.05% in 1873, fell to 5.40% in 1881, rose to 5.88% in 1883, declined to 5% in 1886, and rose again to 7.55% in 1894 at a time of crisis and reforms. These were far above the bond yields then prevailing in European and American financial centers. After 1894, however, Italian yields came down steadily: 4.90% in 1902, 4.80% in 1905, and 3.86% in 1912, based on a new issue of government 31 2s. This was close to the range of government bond yields elsewhere. The yield spread of Italian government bond yields above British consol yields at these dates was as follows:

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The in vivo production of commodity polymers has been limited by the dif culties of controlling these processes, including the control of the reaction conditions required to maintain a bacterial culture and the conditions needed to harvest the polymer product. In addition, the in vivo method, although applicable for a few polymers, is not useful for the full range of polymers produced by the typical chemical processes. An alternate to the in vivo method is the use of enzymes to catalyze in vitro polymerizations. In vitro enzymatic polymerizations are enzyme-catalyzed polymerizations taking place outside cells, that is, taking place in glass and metal reactors. Biochemists carry out in vitro enzymatic polymerizations using the same reactants present in living cells for the purpose of understanding the reactions within living cells [Kamachi et al., 2001]. For polymer chemists, the major effort is the use of enzymes extracted from living cells to carry out in vitro polymerizations of nonnatural substrates to produce polymers for industrial use [Gross et al., 2001; Kobayashi, 1999; Kobayashi et al., 2001]. Lipases are enzymes that catalyze the in vivo hydrolysis of lipids such as triacylglycerols. Lipases are not used in biological systems for ester synthesis, presumably because the large amounts of water present preclude ester formation due to the law of mass action which favors hydrolysis. A different pathway (using the coenzyme A thioester of a carboxylic acid and the enzyme synthase [Blei and Odian, 2000]) is present in biological systems for ester formation. However, lipases do catalyze the in vitro esteri cation reaction and have been used to synthesize polyesters. The reaction between alcohols and carboxylic acids occurs in organic solvents where the absence of water favors esteri cation. However, water is a by-product and must be removed ef ciently to maximize conversions and molecular weights.

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The reaction mechanism for the polymerization of a hydroxyalkanoic acid (Eqs. 2-243 through 2-246) is a chain polymerization, often called an activated monomer polymerization. The active site of lipase is its serine a-amino acid unit, which contains a hydroxyl group. The acyl carbon of the hydroxyalkanoic acid undergoes nucleophilic attack by the hydroxyl group of serine to form lipase-activated monomer (Eq. 2-243). Initiation consists of reaction

2-244 2-243

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